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electrode array microarray format  (CombiMatrix)

 
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    Structured Review

    CombiMatrix electrode array microarray format
    (A) Representative images of an aptamer <t>microarray</t> incubated with labeled IgE. The eight identical subarrays were individually yet simultaneously interrogated with decreasing concentrations of IgE (100 nM to 0.1 nM). Insets (30 nM IgE) demonstrate discrete features of uniform size and intensity. (B,C) Subarrays incubated with 10 nM IgE from two separate experiments were used to analyze intra- and inter-slide data correlation (B and C, respectively). Both correlation analyses demonstrated a high degree of reproducibility, with r 2 values greater than 0.990. The slope of the correlation trend is indicative of relative fluorescence intensities. Slide 2 was washed less stringently than slide 1, hence the higher fluorescence intensities of its features are reflected in a slope of 1.84 (vs. 1.04 in the intraslide comparison where all wash conditions were constant). Averaging of triplicate data points (c, red circles) effectively eliminated outliers seen in the plot of individual data points (black circles). Average percent coefficient of variability (%CV) values for intra- and interslide subarray comparisons were determined to be 5.76% and 6.86%, respectively (see ).
    Electrode Array Microarray Format, supplied by CombiMatrix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/electrode array microarray format/product/CombiMatrix
    Average 90 stars, based on 1 article reviews
    electrode array microarray format - by Bioz Stars, 2026-04
    90/100 stars

    Images

    1) Product Images from "Massively Parallel Interrogation of Aptamer Sequence, Structure and Function"

    Article Title: Massively Parallel Interrogation of Aptamer Sequence, Structure and Function

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0002720

    (A) Representative images of an aptamer microarray incubated with labeled IgE. The eight identical subarrays were individually yet simultaneously interrogated with decreasing concentrations of IgE (100 nM to 0.1 nM). Insets (30 nM IgE) demonstrate discrete features of uniform size and intensity. (B,C) Subarrays incubated with 10 nM IgE from two separate experiments were used to analyze intra- and inter-slide data correlation (B and C, respectively). Both correlation analyses demonstrated a high degree of reproducibility, with r 2 values greater than 0.990. The slope of the correlation trend is indicative of relative fluorescence intensities. Slide 2 was washed less stringently than slide 1, hence the higher fluorescence intensities of its features are reflected in a slope of 1.84 (vs. 1.04 in the intraslide comparison where all wash conditions were constant). Averaging of triplicate data points (c, red circles) effectively eliminated outliers seen in the plot of individual data points (black circles). Average percent coefficient of variability (%CV) values for intra- and interslide subarray comparisons were determined to be 5.76% and 6.86%, respectively (see ).
    Figure Legend Snippet: (A) Representative images of an aptamer microarray incubated with labeled IgE. The eight identical subarrays were individually yet simultaneously interrogated with decreasing concentrations of IgE (100 nM to 0.1 nM). Insets (30 nM IgE) demonstrate discrete features of uniform size and intensity. (B,C) Subarrays incubated with 10 nM IgE from two separate experiments were used to analyze intra- and inter-slide data correlation (B and C, respectively). Both correlation analyses demonstrated a high degree of reproducibility, with r 2 values greater than 0.990. The slope of the correlation trend is indicative of relative fluorescence intensities. Slide 2 was washed less stringently than slide 1, hence the higher fluorescence intensities of its features are reflected in a slope of 1.84 (vs. 1.04 in the intraslide comparison where all wash conditions were constant). Averaging of triplicate data points (c, red circles) effectively eliminated outliers seen in the plot of individual data points (black circles). Average percent coefficient of variability (%CV) values for intra- and interslide subarray comparisons were determined to be 5.76% and 6.86%, respectively (see ).

    Techniques Used: Microarray, Incubation, Labeling, Fluorescence



    Similar Products

    electrode array microarray format  (CombiMatrix)
    90
    CombiMatrix electrode array microarray format
    (A) Representative images of an aptamer <t>microarray</t> incubated with labeled IgE. The eight identical subarrays were individually yet simultaneously interrogated with decreasing concentrations of IgE (100 nM to 0.1 nM). Insets (30 nM IgE) demonstrate discrete features of uniform size and intensity. (B,C) Subarrays incubated with 10 nM IgE from two separate experiments were used to analyze intra- and inter-slide data correlation (B and C, respectively). Both correlation analyses demonstrated a high degree of reproducibility, with r 2 values greater than 0.990. The slope of the correlation trend is indicative of relative fluorescence intensities. Slide 2 was washed less stringently than slide 1, hence the higher fluorescence intensities of its features are reflected in a slope of 1.84 (vs. 1.04 in the intraslide comparison where all wash conditions were constant). Averaging of triplicate data points (c, red circles) effectively eliminated outliers seen in the plot of individual data points (black circles). Average percent coefficient of variability (%CV) values for intra- and interslide subarray comparisons were determined to be 5.76% and 6.86%, respectively (see ).
    Electrode Array Microarray Format, supplied by CombiMatrix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/electrode array microarray format/product/CombiMatrix
    Average 90 stars, based on 1 article reviews
    electrode array microarray format - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Representative images of an aptamer microarray incubated with labeled IgE. The eight identical subarrays were individually yet simultaneously interrogated with decreasing concentrations of IgE (100 nM to 0.1 nM). Insets (30 nM IgE) demonstrate discrete features of uniform size and intensity. (B,C) Subarrays incubated with 10 nM IgE from two separate experiments were used to analyze intra- and inter-slide data correlation (B and C, respectively). Both correlation analyses demonstrated a high degree of reproducibility, with r 2 values greater than 0.990. The slope of the correlation trend is indicative of relative fluorescence intensities. Slide 2 was washed less stringently than slide 1, hence the higher fluorescence intensities of its features are reflected in a slope of 1.84 (vs. 1.04 in the intraslide comparison where all wash conditions were constant). Averaging of triplicate data points (c, red circles) effectively eliminated outliers seen in the plot of individual data points (black circles). Average percent coefficient of variability (%CV) values for intra- and interslide subarray comparisons were determined to be 5.76% and 6.86%, respectively (see ).

    Journal: PLoS ONE

    Article Title: Massively Parallel Interrogation of Aptamer Sequence, Structure and Function

    doi: 10.1371/journal.pone.0002720

    Figure Lengend Snippet: (A) Representative images of an aptamer microarray incubated with labeled IgE. The eight identical subarrays were individually yet simultaneously interrogated with decreasing concentrations of IgE (100 nM to 0.1 nM). Insets (30 nM IgE) demonstrate discrete features of uniform size and intensity. (B,C) Subarrays incubated with 10 nM IgE from two separate experiments were used to analyze intra- and inter-slide data correlation (B and C, respectively). Both correlation analyses demonstrated a high degree of reproducibility, with r 2 values greater than 0.990. The slope of the correlation trend is indicative of relative fluorescence intensities. Slide 2 was washed less stringently than slide 1, hence the higher fluorescence intensities of its features are reflected in a slope of 1.84 (vs. 1.04 in the intraslide comparison where all wash conditions were constant). Averaging of triplicate data points (c, red circles) effectively eliminated outliers seen in the plot of individual data points (black circles). Average percent coefficient of variability (%CV) values for intra- and interslide subarray comparisons were determined to be 5.76% and 6.86%, respectively (see ).

    Article Snippet: Indeed, the Combimatrix electrode array microarray format has the potential to be used much like the Agilent arrays were used here but with an electronic readout.

    Techniques: Microarray, Incubation, Labeling, Fluorescence